Chemical modification of holey carbon supports for cryogenic-electron microscopy
The structural determination of biological molecules has recently undergone a revolution with the Cryogenic-Electron Microscopy (cryo-EM) technique advances. However, a poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits 3D structural determination by S...
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| Format: | Online |
| Language: | English |
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Universidade Estadual de Campinas
2019
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1101 |
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| author | Berenguel, Otavio Portugal, Rodrigo Villares Farias, Marcelo Alexandre de |
| author_facet | Berenguel, Otavio Portugal, Rodrigo Villares Farias, Marcelo Alexandre de |
| author_sort | Berenguel, Otavio |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp |
| description | The structural determination of biological molecules has recently undergone a revolution with the Cryogenic-Electron Microscopy (cryo-EM) technique advances. However, a poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits 3D structural determination by Single Particles Analysis through cryo-EM. This work present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. There is a demonstration of this approach utility to maximize the grid wettability while drive partitioning lipossomes into the holes, thereby enabling better results using cryo-EM. Gold-coated grids with 2-mercaptoethanol yields a hydrophilic surface to holey carbon cryo-EM supports. The method is simple and accessible requiring commercially available items at relatively low costs. The acquired cryo-micrographs for the liposome sample prepared on the SAM grids confirm the success on the drive partitioning of the species into the holes. This approach may enable structure determination of macromolecular targets that suffer from high affinity for holey carbon support. |
| first_indexed | 2025-10-08T13:53:18Z |
| format | Online |
| id | ojs-article-1101 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | eng |
| last_indexed | 2025-10-08T13:53:18Z |
| publishDate | 2019 |
| publisher | Universidade Estadual de Campinas |
| record_format | ojs |
| spelling | ojs-article-11012025-09-26T14:32:30Z Chemical modification of holey carbon supports for cryogenic-electron microscopy Berenguel, Otavio Portugal, Rodrigo Villares Farias, Marcelo Alexandre de Chemical modification Wettability cryo-EM. The structural determination of biological molecules has recently undergone a revolution with the Cryogenic-Electron Microscopy (cryo-EM) technique advances. However, a poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits 3D structural determination by Single Particles Analysis through cryo-EM. This work present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. There is a demonstration of this approach utility to maximize the grid wettability while drive partitioning lipossomes into the holes, thereby enabling better results using cryo-EM. Gold-coated grids with 2-mercaptoethanol yields a hydrophilic surface to holey carbon cryo-EM supports. The method is simple and accessible requiring commercially available items at relatively low costs. The acquired cryo-micrographs for the liposome sample prepared on the SAM grids confirm the success on the drive partitioning of the species into the holes. This approach may enable structure determination of macromolecular targets that suffer from high affinity for holey carbon support. Universidade Estadual de Campinas 2019-02-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1101 10.20396/revpibic2620181101 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp 2596-1969 eng https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1101/1174 Copyright (c) 2019 Otavio Berenguel, Rodrigo Villares Portugal http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Berenguel, Otavio Portugal, Rodrigo Villares Farias, Marcelo Alexandre de Chemical modification Wettability cryo-EM. Chemical modification of holey carbon supports for cryogenic-electron microscopy |
| title | Chemical modification of holey carbon supports for cryogenic-electron microscopy |
| title_full | Chemical modification of holey carbon supports for cryogenic-electron microscopy |
| title_fullStr | Chemical modification of holey carbon supports for cryogenic-electron microscopy |
| title_full_unstemmed | Chemical modification of holey carbon supports for cryogenic-electron microscopy |
| title_short | Chemical modification of holey carbon supports for cryogenic-electron microscopy |
| title_sort | chemical modification of holey carbon supports for cryogenic-electron microscopy |
| topic | Chemical modification Wettability cryo-EM. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1101 |
| work_keys_str_mv | AT berenguelotavio chemicalmodificationofholeycarbonsupportsforcryogenicelectronmicroscopy AT portugalrodrigovillares chemicalmodificationofholeycarbonsupportsforcryogenicelectronmicroscopy AT fariasmarceloalexandrede chemicalmodificationofholeycarbonsupportsforcryogenicelectronmicroscopy |
