Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system
Mutations and deletions of the tumor suppressor LKB1 (STK11) are common in several malignancies, especially in non-small lung cancer cells (NSCLC). In that cell type, LKB1 inactivation coexists with mutations at other important cancer genes, including KRAS and TP53. The mutant LKB1 indirectly increa...
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| Format: | Online |
| Language: | English |
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Universidade Estadual de Campinas
2018
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/138 |
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| author | Morelli, Ana Paula Simabuco, Fernando Moreira Pavan, Isadora Carolina B. Tavares, Mariana Rosolen Salvino, Luiz Guilherme Pereira, Nathalie F. P. Silva, Fernando Riback Junior, Tharcisio Citrangulo Tortelli Chammas, Roger |
| author_facet | Morelli, Ana Paula Simabuco, Fernando Moreira Pavan, Isadora Carolina B. Tavares, Mariana Rosolen Salvino, Luiz Guilherme Pereira, Nathalie F. P. Silva, Fernando Riback Junior, Tharcisio Citrangulo Tortelli Chammas, Roger |
| author_sort | Morelli, Ana Paula |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp |
| description | Mutations and deletions of the tumor suppressor LKB1 (STK11) are common in several malignancies, especially in non-small lung cancer cells (NSCLC). In that cell type, LKB1 inactivation coexists with mutations at other important cancer genes, including KRAS and TP53. The mutant LKB1 indirectly increases transcription of genes involved in metastasis, including COX-2. LKB1 directly activates AMPK decreasing mTOR activity and plays roles in several other processes such as cell metabolism, cell polarity, apoptosis and DNA damage via p53 interaction. Metformin, an antidiabetic drug, have shown antitumor effects, sensitizes cells in coadjuvant cisplatin treatment and acts in AMPK independent and dependent mechanisms. Thus, we propose the application of CRISPR/Cas9 system as genome editing tool to correct the LKB1 nonsense mutation and restore protein expression, providing new insights regarding cisplatin resistance, survival and metformin response in A549 lung cancer cells. |
| first_indexed | 2025-10-08T13:49:35Z |
| format | Online |
| id | ojs-article-138 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | eng |
| last_indexed | 2025-10-08T13:49:35Z |
| publishDate | 2018 |
| publisher | Universidade Estadual de Campinas |
| record_format | ojs |
| spelling | ojs-article-1382025-09-26T14:32:30Z Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system Morelli, Ana Paula Simabuco, Fernando Moreira Pavan, Isadora Carolina B. Tavares, Mariana Rosolen Salvino, Luiz Guilherme Pereira, Nathalie F. P. Silva, Fernando Riback Junior, Tharcisio Citrangulo Tortelli Chammas, Roger CRESPR/Cas9 LKB1 Lung Cancer. Mutations and deletions of the tumor suppressor LKB1 (STK11) are common in several malignancies, especially in non-small lung cancer cells (NSCLC). In that cell type, LKB1 inactivation coexists with mutations at other important cancer genes, including KRAS and TP53. The mutant LKB1 indirectly increases transcription of genes involved in metastasis, including COX-2. LKB1 directly activates AMPK decreasing mTOR activity and plays roles in several other processes such as cell metabolism, cell polarity, apoptosis and DNA damage via p53 interaction. Metformin, an antidiabetic drug, have shown antitumor effects, sensitizes cells in coadjuvant cisplatin treatment and acts in AMPK independent and dependent mechanisms. Thus, we propose the application of CRISPR/Cas9 system as genome editing tool to correct the LKB1 nonsense mutation and restore protein expression, providing new insights regarding cisplatin resistance, survival and metformin response in A549 lung cancer cells. Universidade Estadual de Campinas 2018-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/138 10.20396/revpibic262018138 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp 2596-1969 eng https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/138/117 Copyright (c) 2018 Ana Paula Morelli, Fernando Moreira Simabuco http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Morelli, Ana Paula Simabuco, Fernando Moreira Pavan, Isadora Carolina B. Tavares, Mariana Rosolen Salvino, Luiz Guilherme Pereira, Nathalie F. P. Silva, Fernando Riback Junior, Tharcisio Citrangulo Tortelli Chammas, Roger CRESPR/Cas9 LKB1 Lung Cancer. Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system |
| title | Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system |
| title_full | Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system |
| title_fullStr | Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system |
| title_full_unstemmed | Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system |
| title_short | Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system |
| title_sort | genome editing of lkb1 (stk11) mutation in a549 lung cancer cells using crispr/cas9 system |
| topic | CRESPR/Cas9 LKB1 Lung Cancer. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/138 |
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