Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology
The marine amphipod Parhyale hawaiensis is able to regenerate its appendages, limbs and tissues. The objective of this study was: (i) verify whether the regeneration of P. hawaiensis antenna can be used as an endpoint in a miniaturized toxicity test system (12 well microplates, 5 mL) and, later, (ii...
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| Format: | Online |
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Universidade Estadual de Campinas
2019
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1701 |
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| author | Diehl, Otavio Umbuzeiro, Gisela Assano, Patrícia Oliveira, Rhaul de |
| author_facet | Diehl, Otavio Umbuzeiro, Gisela Assano, Patrícia Oliveira, Rhaul de |
| author_sort | Diehl, Otavio |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 27 (2019): Congresso de Iniciação Científica UNICAMP; 1-1 |
| description | The marine amphipod Parhyale hawaiensis is able to regenerate its appendages, limbs and tissues. The objective of this study was: (i) verify whether the regeneration of P. hawaiensis antenna can be used as an endpoint in a miniaturized toxicity test system (12 well microplates, 5 mL) and, later, (ii) apply this endpoint in the toxicity assessment of dimethyl sulfoxide (DMSO), a organic solvent commonly used in toxicity tests, and diflubenzuron (DFB), a growth regulator insecticide. The average regeneration time of fifty per cent of the population (RT50) in reconstituted saltwater tests was 16.7 days. DMSO induced the regeneration concentracions above 0.02%. As expected for chitin syntesis inhibitor, DFB strogly inhibited the antenna regeneration with a 28 d-EC50 = 0.5 ?g/L. Thefore, we conclude that regeneration time can be used as endpoint in a cost effective miniaturized toxicity test, with time < 35 d. Moreover, DMSO use is not indicate. |
| first_indexed | 2025-10-08T13:55:05Z |
| format | Online |
| id | ojs-article-1701 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | por |
| last_indexed | 2025-10-08T13:55:05Z |
| publishDate | 2019 |
| publisher | Universidade Estadual de Campinas |
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| spelling | ojs-article-17012025-09-26T14:39:16Z Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology Diehl, Otavio Umbuzeiro, Gisela Assano, Patrícia Oliveira, Rhaul de Marine toxicology Growth inhibitors Regeneration The marine amphipod Parhyale hawaiensis is able to regenerate its appendages, limbs and tissues. The objective of this study was: (i) verify whether the regeneration of P. hawaiensis antenna can be used as an endpoint in a miniaturized toxicity test system (12 well microplates, 5 mL) and, later, (ii) apply this endpoint in the toxicity assessment of dimethyl sulfoxide (DMSO), a organic solvent commonly used in toxicity tests, and diflubenzuron (DFB), a growth regulator insecticide. The average regeneration time of fifty per cent of the population (RT50) in reconstituted saltwater tests was 16.7 days. DMSO induced the regeneration concentracions above 0.02%. As expected for chitin syntesis inhibitor, DFB strogly inhibited the antenna regeneration with a 28 d-EC50 = 0.5 ?g/L. Thefore, we conclude that regeneration time can be used as endpoint in a cost effective miniaturized toxicity test, with time < 35 d. Moreover, DMSO use is not indicate. Universidade Estadual de Campinas 2019-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1701 10.20396/revpibic2720191701 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 27 (2019): Congresso de Iniciação Científica UNICAMP; 1-1 2596-1969 por https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1701/1776 Copyright (c) 2019 Revista dos Trabalhos de Iniciação Científica da UNICAMP http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Diehl, Otavio Umbuzeiro, Gisela Assano, Patrícia Oliveira, Rhaul de Marine toxicology Growth inhibitors Regeneration Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology |
| title | Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology |
| title_full | Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology |
| title_fullStr | Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology |
| title_full_unstemmed | Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology |
| title_short | Antennae regeneration of marine amphipod Parhyale Hawaiensis as endpoint in ecotoxicology |
| title_sort | antennae regeneration of marine amphipod parhyale hawaiensis as endpoint in ecotoxicology |
| topic | Marine toxicology Growth inhibitors Regeneration |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/1701 |
| work_keys_str_mv | AT diehlotavio antennaeregenerationofmarineamphipodparhyalehawaiensisasendpointinecotoxicology AT umbuzeirogisela antennaeregenerationofmarineamphipodparhyalehawaiensisasendpointinecotoxicology AT assanopatricia antennaeregenerationofmarineamphipodparhyalehawaiensisasendpointinecotoxicology AT oliveirarhaulde antennaeregenerationofmarineamphipodparhyalehawaiensisasendpointinecotoxicology |
