Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans
Periodontal ligament (PDL) is constituted by heterogeneous cell populations with different cell differentiation capacities, here we investigated the effect of an epigenetic modulator on the induction of osteo/cementoblastic differentiation of human periodontal ligament mesenchymal progenitor cell cl...
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| Language: | Portuguese |
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Universidade Estadual de Campinas
2019
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| author | Santos, Karoline dos Ruiz, Karina Sacramento, Catharina |
| author_facet | Santos, Karoline dos Ruiz, Karina Sacramento, Catharina |
| author_sort | Santos, Karoline dos |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 27 (2019): Congresso de Iniciação Científica UNICAMP; 1-1 |
| description | Periodontal ligament (PDL) is constituted by heterogeneous cell populations with different cell differentiation capacities, here we investigated the effect of an epigenetic modulator on the induction of osteo/cementoblastic differentiation of human periodontal ligament mesenchymal progenitor cell clones with positive surface markers for CD105 (hPDLCD105+) and with low osteo/cementoblastic potential (LOP). These clones were pretreated with 5-aza-2'-deoxycytidine (5-aza-dC) and subjected to mineralization assays (Alizarin Red and Xylenol Orange), transcript levels’ analysis of ALP, ANXA2, ASPN, BMP2, FGF7, OCN, OPN and RUNX2, using qRT-PCR. As results, 5-aza-dC enhances the capacity of mineralized matrix deposition of two LOP clones (L1 and L2), however different results regarding to the quantity of calcium nodules production was found, where L1 mineralization and osteogenic-related gene expression was greater than L2. The data show that the augmentation of BMP2, FGF7 and ANXA2 transcript levels may lead to a better osteo/cementogenic response, while the elevation of ASPN gene expression may block the mineralization performance of LOP clones. These results showed that pre-treatment with 5-aza-dC can positively modulate the osteo/cementogenic differentiation of hPDL-CD105+ cell clones with low osteo/ emento-blastic potential. Those findings contribute to better understand the complex mechanisms that can lead PDL stem cells to regenerate the periodontal tissues. |
| first_indexed | 2025-10-08T13:57:13Z |
| format | Online |
| id | ojs-article-2363 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | por |
| last_indexed | 2025-10-08T13:57:13Z |
| publishDate | 2019 |
| publisher | Universidade Estadual de Campinas |
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| spelling | ojs-article-23632025-09-26T14:39:16Z Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans Santos, Karoline dos Ruiz, Karina Sacramento, Catharina Cell differentiation Gene expression Stem cells Periodontal ligament (PDL) is constituted by heterogeneous cell populations with different cell differentiation capacities, here we investigated the effect of an epigenetic modulator on the induction of osteo/cementoblastic differentiation of human periodontal ligament mesenchymal progenitor cell clones with positive surface markers for CD105 (hPDLCD105+) and with low osteo/cementoblastic potential (LOP). These clones were pretreated with 5-aza-2'-deoxycytidine (5-aza-dC) and subjected to mineralization assays (Alizarin Red and Xylenol Orange), transcript levels’ analysis of ALP, ANXA2, ASPN, BMP2, FGF7, OCN, OPN and RUNX2, using qRT-PCR. As results, 5-aza-dC enhances the capacity of mineralized matrix deposition of two LOP clones (L1 and L2), however different results regarding to the quantity of calcium nodules production was found, where L1 mineralization and osteogenic-related gene expression was greater than L2. The data show that the augmentation of BMP2, FGF7 and ANXA2 transcript levels may lead to a better osteo/cementogenic response, while the elevation of ASPN gene expression may block the mineralization performance of LOP clones. These results showed that pre-treatment with 5-aza-dC can positively modulate the osteo/cementogenic differentiation of hPDL-CD105+ cell clones with low osteo/ emento-blastic potential. Those findings contribute to better understand the complex mechanisms that can lead PDL stem cells to regenerate the periodontal tissues. Universidade Estadual de Campinas 2019-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2363 10.20396/revpibic2720192363 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 27 (2019): Congresso de Iniciação Científica UNICAMP; 1-1 2596-1969 por https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2363/2404 Copyright (c) 2019 Revista dos Trabalhos de Iniciação Científica da UNICAMP http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Santos, Karoline dos Ruiz, Karina Sacramento, Catharina Cell differentiation Gene expression Stem cells Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| title | Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| title_full | Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| title_fullStr | Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| title_full_unstemmed | Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| title_short | Evaluation of 5-Azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| title_sort | evaluation of 5-azacytidine as an inducer of osteoblastic / cementoblastic differentiation of progenitor cells from the periodontal ligament of humans |
| topic | Cell differentiation Gene expression Stem cells |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2363 |
| work_keys_str_mv | AT santoskarolinedos evaluationof5azacytidineasaninducerofosteoblasticcementoblasticdifferentiationofprogenitorcellsfromtheperiodontalligamentofhumans AT ruizkarina evaluationof5azacytidineasaninducerofosteoblasticcementoblasticdifferentiationofprogenitorcellsfromtheperiodontalligamentofhumans AT sacramentocatharina evaluationof5azacytidineasaninducerofosteoblasticcementoblasticdifferentiationofprogenitorcellsfromtheperiodontalligamentofhumans |
