Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S)
The aim of this study was to determine the hydrodynamic parameters of ?-lactamase OXA-143(P227S) and to evaluate the effect of sulfate and bicarbonate salts on the penicillinase activity of the enzyme. Using chromatographic and spectroscopic techniques, it was possible to determine its diffusion coe...
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| Format: | Online |
| Language: | Portuguese |
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Universidade Estadual de Campinas
2019
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2601 |
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| author | Rospendowski, Rafael Favaro, Denize Antunes, Víctor |
| author_facet | Rospendowski, Rafael Favaro, Denize Antunes, Víctor |
| author_sort | Rospendowski, Rafael |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 27 (2019): Congresso de Iniciação Científica UNICAMP; 1-1 |
| description | The aim of this study was to determine the hydrodynamic parameters of ?-lactamase OXA-143(P227S) and to evaluate the effect of sulfate and bicarbonate salts on the penicillinase activity of the enzyme. Using chromatographic and spectroscopic techniques, it was possible to determine its diffusion coefficient, hydrodynamic radius and molecular mass, showing that mutant P227S has a tertiary structure of globular shape. The kinetic results showed an increase in catalytic activity only in the assay with increasing bicarbonate concentration. The bicarbonate supplementation also increases the thermal stability of the enzyme, which doesn't occur with the addition of sulfate. |
| first_indexed | 2025-10-08T13:57:57Z |
| format | Online |
| id | ojs-article-2601 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | por |
| last_indexed | 2025-10-08T13:57:57Z |
| publishDate | 2019 |
| publisher | Universidade Estadual de Campinas |
| record_format | ojs |
| spelling | ojs-article-26012025-09-26T14:39:16Z Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) Rospendowski, Rafael Favaro, Denize Antunes, Víctor Antibiotic resistance β-lactamase Enzyme kinetics The aim of this study was to determine the hydrodynamic parameters of ?-lactamase OXA-143(P227S) and to evaluate the effect of sulfate and bicarbonate salts on the penicillinase activity of the enzyme. Using chromatographic and spectroscopic techniques, it was possible to determine its diffusion coefficient, hydrodynamic radius and molecular mass, showing that mutant P227S has a tertiary structure of globular shape. The kinetic results showed an increase in catalytic activity only in the assay with increasing bicarbonate concentration. The bicarbonate supplementation also increases the thermal stability of the enzyme, which doesn't occur with the addition of sulfate. Universidade Estadual de Campinas 2019-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2601 10.20396/revpibic2720192601 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 27 (2019): Congresso de Iniciação Científica UNICAMP; 1-1 2596-1969 por https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2601/2615 Copyright (c) 2019 Revista dos Trabalhos de Iniciação Científica da UNICAMP http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Rospendowski, Rafael Favaro, Denize Antunes, Víctor Antibiotic resistance β-lactamase Enzyme kinetics Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) |
| title | Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) |
| title_full | Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) |
| title_fullStr | Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) |
| title_full_unstemmed | Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) |
| title_short | Characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase OXA-143 (P227S) |
| title_sort | characterization and study of the effect of sulfate and bicarbonate on the catalytic activity of ?-lactamase oxa-143 (p227s) |
| topic | Antibiotic resistance β-lactamase Enzyme kinetics |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2601 |
| work_keys_str_mv | AT rospendowskirafael characterizationandstudyoftheeffectofsulfateandbicarbonateonthecatalyticactivityoflactamaseoxa143p227s AT favarodenize characterizationandstudyoftheeffectofsulfateandbicarbonateonthecatalyticactivityoflactamaseoxa143p227s AT antunesvictor characterizationandstudyoftheeffectofsulfateandbicarbonateonthecatalyticactivityoflactamaseoxa143p227s |
