Selection of wild-type S. cerevisiae strains tolerant to the presence of n-butanol from evolutionary engineering

Butanol is a biofuel considered to be the best substitute for gasoline when compared to ethanol. The production of n-butanol from S. cerevisiae is still a challenge due to the low final concentration produced and low tolerance to this alcohol. The objective of this project was to select wild-type st...

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Bibliographic Details
Main Authors: Inoue, Heitor, Goldbeck, Rosana, Azambuja, Suéllen
Format: Online
Language:Portuguese
Published: Universidade Estadual de Campinas 2019
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Online Access:https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/2901
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Summary:Butanol is a biofuel considered to be the best substitute for gasoline when compared to ethanol. The production of n-butanol from S. cerevisiae is still a challenge due to the low final concentration produced and low tolerance to this alcohol. The objective of this project was to select wild-type strains of S. cerevisiae tolerant to the presence of n-butanol, to carry out evolutionary engineering tests, aiming to increase the tolerance to n-butanol, and to characterize the selected colonies. Two strains were studied, and colonies more tolerant to n-butanol were obtained through evolutionary engineering.
ISSN:2596-1969