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Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population

This project aims to analyze the HLA alleles frequency in the Brazilian population. We used DNA samples of 36 healthy Brazilians subjects to sequence classic genes of the HLA class I (HLA-A, -B and -C) and class II (HLA-DQA1, -DQB1, -DPA1, -DPB1 and -DRB1, 3, 4 and 5) using next-generation sequencin...

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Bibliographic Details
Main Authors: Rosa, Douglas Cescon da, Cendes, Iscia Teresinha Lopes, Araujo, Tânia Kawazaki de, Torres, Fábio Rossi, Cendes, Fernando, Watanabe, Nancy
Format: Online
Language:English
Published: Universidade Estadual de Campinas 2018
Subjects:
Next-generation sequencing
HLA system
Allelic frequency.
Online Access:https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/360
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Summary:This project aims to analyze the HLA alleles frequency in the Brazilian population. We used DNA samples of 36 healthy Brazilians subjects to sequence classic genes of the HLA class I (HLA-A, -B and -C) and class II (HLA-DQA1, -DQB1, -DPA1, -DPB1 and -DRB1, 3, 4 and 5) using next-generation sequencing (NGS). We used the HLA TruSight v2 Illumina kit to prepare HLA amplicons of these genes and the libraries generated were subsequently sequencied in an Illumina equipament, The FASTQ data generated by sequencing were analyzed by the TruSight Assign HLA (Illumina) software. The data generated in this study will be made publicly available in the BIPMed project (www.bipmed.org), so it can be used as a reference for further studies in the Brazilian population.
ISSN:2596-1969

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