Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population
This project aims to analyze the HLA alleles frequency in the Brazilian population. We used DNA samples of 36 healthy Brazilians subjects to sequence classic genes of the HLA class I (HLA-A, -B and -C) and class II (HLA-DQA1, -DQB1, -DPA1, -DPB1 and -DRB1, 3, 4 and 5) using next-generation sequencin...
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| Format: | Online |
| Language: | English |
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Universidade Estadual de Campinas
2018
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/360 |
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| author | Rosa, Douglas Cescon da Cendes, Iscia Teresinha Lopes Araujo, Tânia Kawazaki de Torres, Fábio Rossi Cendes, Fernando Watanabe, Nancy |
| author_facet | Rosa, Douglas Cescon da Cendes, Iscia Teresinha Lopes Araujo, Tânia Kawazaki de Torres, Fábio Rossi Cendes, Fernando Watanabe, Nancy |
| author_sort | Rosa, Douglas Cescon da |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp |
| description | This project aims to analyze the HLA alleles frequency in the Brazilian population. We used DNA samples of 36 healthy Brazilians subjects to sequence classic genes of the HLA class I (HLA-A, -B and -C) and class II (HLA-DQA1, -DQB1, -DPA1, -DPB1 and -DRB1, 3, 4 and 5) using next-generation sequencing (NGS). We used the HLA TruSight v2 Illumina kit to prepare HLA amplicons of these genes and the libraries generated were subsequently sequencied in an Illumina equipament, The FASTQ data generated by sequencing were analyzed by the TruSight Assign HLA (Illumina) software. The data generated in this study will be made publicly available in the BIPMed project (www.bipmed.org), so it can be used as a reference for further studies in the Brazilian population. |
| first_indexed | 2025-10-08T13:50:33Z |
| format | Online |
| id | ojs-article-360 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | eng |
| last_indexed | 2025-10-08T13:50:33Z |
| publishDate | 2018 |
| publisher | Universidade Estadual de Campinas |
| record_format | ojs |
| spelling | ojs-article-3602025-09-26T14:32:30Z Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population Rosa, Douglas Cescon da Cendes, Iscia Teresinha Lopes Araujo, Tânia Kawazaki de Torres, Fábio Rossi Cendes, Fernando Watanabe, Nancy Next-generation sequencing HLA system Allelic frequency. This project aims to analyze the HLA alleles frequency in the Brazilian population. We used DNA samples of 36 healthy Brazilians subjects to sequence classic genes of the HLA class I (HLA-A, -B and -C) and class II (HLA-DQA1, -DQB1, -DPA1, -DPB1 and -DRB1, 3, 4 and 5) using next-generation sequencing (NGS). We used the HLA TruSight v2 Illumina kit to prepare HLA amplicons of these genes and the libraries generated were subsequently sequencied in an Illumina equipament, The FASTQ data generated by sequencing were analyzed by the TruSight Assign HLA (Illumina) software. The data generated in this study will be made publicly available in the BIPMed project (www.bipmed.org), so it can be used as a reference for further studies in the Brazilian population. Universidade Estadual de Campinas 2018-12-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/360 10.20396/revpibic262018360 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp 2596-1969 eng https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/360/344 Copyright (c) 2018 Douglas Cescon da Rosa, Iscia Teresinha Lopes Cendes http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Rosa, Douglas Cescon da Cendes, Iscia Teresinha Lopes Araujo, Tânia Kawazaki de Torres, Fábio Rossi Cendes, Fernando Watanabe, Nancy Next-generation sequencing HLA system Allelic frequency. Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population |
| title | Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population |
| title_full | Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population |
| title_fullStr | Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population |
| title_full_unstemmed | Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population |
| title_short | Analysis of the human leukocitary antigen (HLA) system distribution in the brazilian population |
| title_sort | analysis of the human leukocitary antigen (hla) system distribution in the brazilian population |
| topic | Next-generation sequencing HLA system Allelic frequency. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/360 |
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