Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role
Thyroid carcinoma is the most common endocrine cancer in the world. Over the last years attention has been focused on the role of microRNAs (miRs), small non-coding RNAs molecules, in the tumoral thyroid cell biology, especially in the diagnosis and prognosis of the papillary histotype (PTC). In a r...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Universidade Estadual de Campinas
2018
|
| Subjects: | |
| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/59 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864358322838175744 |
|---|---|
| author | Alves, Letícia Ferreira Geraldo, Murilo Vieira Marson, Leonardo Augusto |
| author_facet | Alves, Letícia Ferreira Geraldo, Murilo Vieira Marson, Leonardo Augusto |
| author_sort | Alves, Letícia Ferreira |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp |
| description | Thyroid carcinoma is the most common endocrine cancer in the world. Over the last years attention has been focused on the role of microRNAs (miRs), small non-coding RNAs molecules, in the tumoral thyroid cell biology, especially in the diagnosis and prognosis of the papillary histotype (PTC). In a recent study of the group, the expression of miRs from the DLK1-DIO3 genomic region has been shown to be decreased in PTC. Importantly, distinct members from this genomic region have already been described as tumor suppressor miRs in several types of cancer. The aim of the project herein was to identify, based on target prediction and gene enrichment analysis, candidate miRs with tumor suppressor potential and to generate DNA plasmid constructions. MiR-495-3p and miR-485-5p were identified as the candidates with the highest tumor suppressor potential role in PTC among more than 50 miRs from the DLK1-DIO3 region. The construction of expression systems containing the miRs was performed based in mollecular clonning methodology and will certainly contribute to further functional validation of the role of these miRs in PTC. |
| first_indexed | 2025-10-08T13:49:41Z |
| format | Online |
| id | ojs-article-59 |
| institution | Universidade Estadual de Campinas |
| issn | 2596-1969 |
| language | eng |
| last_indexed | 2025-10-08T13:49:41Z |
| publishDate | 2018 |
| publisher | Universidade Estadual de Campinas |
| record_format | ojs |
| spelling | ojs-article-592025-09-26T14:32:30Z Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role Alves, Letícia Ferreira Geraldo, Murilo Vieira Marson, Leonardo Augusto Molecular clonning Thyroid cancer MicroRNAs. Thyroid carcinoma is the most common endocrine cancer in the world. Over the last years attention has been focused on the role of microRNAs (miRs), small non-coding RNAs molecules, in the tumoral thyroid cell biology, especially in the diagnosis and prognosis of the papillary histotype (PTC). In a recent study of the group, the expression of miRs from the DLK1-DIO3 genomic region has been shown to be decreased in PTC. Importantly, distinct members from this genomic region have already been described as tumor suppressor miRs in several types of cancer. The aim of the project herein was to identify, based on target prediction and gene enrichment analysis, candidate miRs with tumor suppressor potential and to generate DNA plasmid constructions. MiR-495-3p and miR-485-5p were identified as the candidates with the highest tumor suppressor potential role in PTC among more than 50 miRs from the DLK1-DIO3 region. The construction of expression systems containing the miRs was performed based in mollecular clonning methodology and will certainly contribute to further functional validation of the role of these miRs in PTC. Universidade Estadual de Campinas 2018-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo de convidado application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/59 10.20396/revpibic26201859 Revista dos Trabalhos de Iniciação Científica da UNICAMP; n. 26 (2018): Congresso de Iniciação Científica Unicamp 2596-1969 eng https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/59/222 Copyright (c) 2018 Letícia Ferreira Alves, Murilo Vieira Geraldo http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Alves, Letícia Ferreira Geraldo, Murilo Vieira Marson, Leonardo Augusto Molecular clonning Thyroid cancer MicroRNAs. Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role |
| title | Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role |
| title_full | Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role |
| title_fullStr | Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role |
| title_full_unstemmed | Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role |
| title_short | Construction of constitutive expression systems of microRNAs from the genomic region DLK1-DIO3 with putative tumor suppressor role |
| title_sort | construction of constitutive expression systems of micrornas from the genomic region dlk1-dio3 with putative tumor suppressor role |
| topic | Molecular clonning Thyroid cancer MicroRNAs. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/pibic/article/view/59 |
| work_keys_str_mv | AT alvesleticiaferreira constructionofconstitutiveexpressionsystemsofmicrornasfromthegenomicregiondlk1dio3withputativetumorsuppressorrole AT geraldomurilovieira constructionofconstitutiveexpressionsystemsofmicrornasfromthegenomicregiondlk1dio3withputativetumorsuppressorrole AT marsonleonardoaugusto constructionofconstitutiveexpressionsystemsofmicrornasfromthegenomicregiondlk1dio3withputativetumorsuppressorrole |
