Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS
The international program "ICLAS Animal Quality Network" was developed in 2006 with the goal of improving the quality of animals used in scientific researches. The "Performance Evaluation Program” (PEP)" was created to assist diagnostic laboratories in evaluating the sensitivity...
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Universidade Estadual de Campinas
2016
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| author | Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson |
| author_facet | Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson |
| author_sort | Moreira, Josélia Cristina de Oliveira |
| collection | Portal da Incubadora de Periódicos Científicos, Acadêmicos e Educacionais |
| container_reference | Revista Saberes Universitários; Vol. 1 No. 1 (2016); 50-62 |
| description | The international program "ICLAS Animal Quality Network" was developed in 2006 with the goal of improving the quality of animals used in scientific researches. The "Performance Evaluation Program” (PEP)" was created to assist diagnostic laboratories in evaluating the sensitivity and specificity of laboratory tests. The program has 22 participants worldwide, and the CEMIB / UNICAMP Animal Health Laboratory is the only one in South America that attends this program. For analysis, were received 20 blinded samples: isolated bacterial cultures of intestinal fluid and vaginal tract, feces of mice, lung and spleen homogenates, 5 mice and 5 rat sera. For bacteriological diagnosis, selective and differential medium were used. Sera were tested by Indirect Immunofluorescence assay for 16 antigens, and the PCR and RT-PCR were applied for tissue samples. From this total, the laboratory correctly identified 17 of them, which corresponds to 89.4% of compatibility in the diagnosis. The bacteria identified were: Klebisiella oxytoca, Streptococcus agalactiae, Stenotrophomonas malthophilia, Staphylococcus sciuri, and Mycoplasma pulmonis. It was observed that the serum had specific antibodies to: Reovirus-3, Mouse minute virus, Mycoplasma pulmonis, Murine hepatitis virus, Sendai virus, rat coronavirus, rat theilovirus and Adenovirus. Two of these sera showed negative results for the tested antigens. Molecular analyses of lung and spleen homogenates were positive for Parvovirus and Pneumocystis murina, respectively. This first trial was important to evaluate the methodologies adopted and to detect potential problems related to methods used in the process, aiming to ensure the quality of the laboratory diagnosis offered to the scientific community |
| first_indexed | 2025-10-08T14:36:26Z |
| format | Online |
| id | ojs-article-6950 |
| institution | Universidade Estadual de Campinas |
| issn | 2447-9411 |
| language | por |
| last_indexed | 2026-05-28T21:01:45Z |
| publishDate | 2016 |
| publisher | Universidade Estadual de Campinas |
| record_format | ojs |
| spelling | ojs-article-69502026-05-28T16:59:39Z Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS Certificação de métodos microbiológicos de diagnóstico do Laboratório de Controle Qualidade Sanitária do CEMIB/UNICAMP junto ao ICLAS Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson ICLAS Sanitary monitoring Indirect Immunofluorescence assay PCR RT-PCR ICLAS Monitoramento sanitário Imunofluorescencia indireta PCR RT-PCR The international program "ICLAS Animal Quality Network" was developed in 2006 with the goal of improving the quality of animals used in scientific researches. The "Performance Evaluation Program” (PEP)" was created to assist diagnostic laboratories in evaluating the sensitivity and specificity of laboratory tests. The program has 22 participants worldwide, and the CEMIB / UNICAMP Animal Health Laboratory is the only one in South America that attends this program. For analysis, were received 20 blinded samples: isolated bacterial cultures of intestinal fluid and vaginal tract, feces of mice, lung and spleen homogenates, 5 mice and 5 rat sera. For bacteriological diagnosis, selective and differential medium were used. Sera were tested by Indirect Immunofluorescence assay for 16 antigens, and the PCR and RT-PCR were applied for tissue samples. From this total, the laboratory correctly identified 17 of them, which corresponds to 89.4% of compatibility in the diagnosis. The bacteria identified were: Klebisiella oxytoca, Streptococcus agalactiae, Stenotrophomonas malthophilia, Staphylococcus sciuri, and Mycoplasma pulmonis. It was observed that the serum had specific antibodies to: Reovirus-3, Mouse minute virus, Mycoplasma pulmonis, Murine hepatitis virus, Sendai virus, rat coronavirus, rat theilovirus and Adenovirus. Two of these sera showed negative results for the tested antigens. Molecular analyses of lung and spleen homogenates were positive for Parvovirus and Pneumocystis murina, respectively. This first trial was important to evaluate the methodologies adopted and to detect potential problems related to methods used in the process, aiming to ensure the quality of the laboratory diagnosis offered to the scientific community O programa internacional “ICLAS Animal Quality Network” foi desenvolvido em 2007 com o objetivo de melhorar a qualidade dos animais utilizados em pesquisas científicas. Desta forma, foi criado o “Performance Evaluation Program (PEP)”para auxiliar laboratórios de diagnóstico na avaliação da sensibilidade e especificidade dos testes laboratoriais. O programa conta com 22 participantes mundiais e na América do Sul apenas o Laboratório de Controle de Qualidade Sanitária do CEMIB/UNICAMP faz parte deste grupo. Foram recebidas 20 amostras biológicas “cegas” constituídas de: culturas bacterianas isoladas de trato intestinal, fluído e vagina; fezes de camundongo; homogenato de pulmão e baço; 5 soros de ratos e 5 soros de camundongo. Para diagnóstico bacteriológico foram utilizados meios de cultivos de enriquecimento e seletivos-diferenciais. Os soros foram testados por Imunofluorescência Indireta para 16 antígenos virais e as amostras de tecido por PCR e RT-PCR. Das 20 amostras, o laboratório identificou corretamente 17 delas, que corresponde a 89,4% de compatibilidade no diagnóstico. As bactérias identificadas foram: Klebisiella oxytoca, Streptococcus agalactiae, Stenotrophomonas malthophilia, Staphylococcus sciuri e Mycoplasma pulmonis. Os soros apresentaram anticorpos contra: Reovírus-3, vírus minuto do camundongo, Mycoplasma pulmonis, vírus da Hepatite murina, vírus Sendai, Rat coronavírus, Rat Theilovírus e Adenovírus. Destes soros 2 foram negativos. As análises moleculares do homogenato de pulmão e baço apresentaram positividade para Pneumocystis murina e Parvovírus, respectivamente. Esta primeira triagem foi importante para avaliar as metodologias adotadas e detectar possíveis problemas relacionados aos métodos utilizados no processo, garantindo a certificação da qualidade do diagnóstico oferecido pelo laboratório à comunidade científica. Universidade Estadual de Campinas 2016-03-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://econtents.sbu.unicamp.br/inpec/index.php/saberes/article/view/6950 10.20396/saberes.v1i1.6950 Revista Saberes Universitários; Vol. 1 No. 1 (2016); 50-62 Revista Saberes Universitários; v. 1 n. 1 (2016); 50-62 2447-9411 por https://econtents.sbu.unicamp.br/inpec/index.php/saberes/article/view/6950/2442 Brazil; Contemporany Brasil; Contemporâneo Copyright (c) 2016 Saberes Universitários https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson Moreira, Josélia Cristina de Oliveira Demolin, Daniele Masselli Rodrigues Issei, Clarice Yukari Minagawa Santos, Silvio Rogério Cardoso dos Andrade, Lenira Aparecida Guaraldo de Silva, Euma Cunha Ramos Gilioli, Rovilson ICLAS Sanitary monitoring Indirect Immunofluorescence assay PCR RT-PCR ICLAS Monitoramento sanitário Imunofluorescencia indireta PCR RT-PCR Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS |
| title | Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS |
| title_full | Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS |
| title_fullStr | Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS |
| title_full_unstemmed | Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS |
| title_short | Certification of microbiological methods of diagnosis of Health Quality Control Laboratory CEMIB / UNICAMP with the ICLAS |
| title_sort | certification of microbiological methods of diagnosis of health quality control laboratory cemib / unicamp with the iclas |
| topic | ICLAS Sanitary monitoring Indirect Immunofluorescence assay PCR RT-PCR ICLAS Monitoramento sanitário Imunofluorescencia indireta PCR RT-PCR |
| url | https://econtents.sbu.unicamp.br/inpec/index.php/saberes/article/view/6950 |
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