High pressure effect on the pharmaceutical substances efficiency
Some pathogenic bacteria manufacture -lactamases, enzymes that hydrolyze the antibiotic -lactam rings, such as penicillin and cephalosporin. Currently, the search for anti -lactam substances is still significantly important. Recent researches point out clavulanic acid (CA) as the main -lactam compou...
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| Format: | Online |
| Language: | Portuguese |
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Universidade Estadual de Campinas (UNICAMP)
2016
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/8875 |
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| Summary: | Some pathogenic bacteria manufacture -lactamases, enzymes that hydrolyze the antibiotic -lactam rings, such as penicillin and cephalosporin. Currently, the search for anti -lactam substances is still significantly important. Recent researches point out clavulanic acid (CA) as the main -lactam compound manufactured by Streptomyces clavuligerus. One of this biomolecule characteristics is the instability, due to the environment conditions where it is, affecting the purification yield. In this context, this work presents a CA stability study in different temperature (T), pH, buffer concentration (BC) and pressure (P) conditions. The CA solutions were submitted to different conditions for a defined time span and the concentrations were followed up, determining the answer, which is the hydrolysis constant (k). The experiments were performed in a cell with magnetic stirring and T and P controlled by thermostatically-controlled water bath and gaseous nitrogen injection, respectively. The preliminary tests enable to deduce on the influence of all factors, though they don't enable to assess the interaction between them. The results confirm literature information regarding the temperature and buffer concentration influence: the hydrolysis constant was higher at 34 ºC than at 17 ºC (pH 6.0, CT 500 mM) and at 500 mM than at 20 mM (17 ºC, pH 4.5). In addition to that, they point out to the unexpected possibility that the pressure increase is unfavorable to the hydrolysis constant. |
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| ISSN: | 2525-5398 |
