High pressure effect on the pharmaceutical substances efficiency
Some pathogenic bacteria manufacture -lactamases, enzymes that hydrolyze the antibiotic -lactam rings, such as penicillin and cephalosporin. Currently, the search for anti -lactam substances is still significantly important. Recent researches point out clavulanic acid (CA) as the main -lactam compou...
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Universidade Estadual de Campinas (UNICAMP)
2016
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| author | Carvalho, Priscila Hoffmann |
| author_facet | Carvalho, Priscila Hoffmann |
| author_sort | Carvalho, Priscila Hoffmann |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Resumo dos trabalhos do SIMTEC Simpósio dos Profissionais da UNICAMP; v. 6 (2016): Temática: UNICAMP 50 ANOS - Memórias, Experiências e Trajetórias profissionais; 144-144 |
| description | Some pathogenic bacteria manufacture -lactamases, enzymes that hydrolyze the antibiotic -lactam rings, such as penicillin and cephalosporin. Currently, the search for anti -lactam substances is still significantly important. Recent researches point out clavulanic acid (CA) as the main -lactam compound manufactured by Streptomyces clavuligerus. One of this biomolecule characteristics is the instability, due to the environment conditions where it is, affecting the purification yield. In this context, this work presents a CA stability study in different temperature (T), pH, buffer concentration (BC) and pressure (P) conditions. The CA solutions were submitted to different conditions for a defined time span and the concentrations were followed up, determining the answer, which is the hydrolysis constant (k). The experiments were performed in a cell with magnetic stirring and T and P controlled by thermostatically-controlled water bath and gaseous nitrogen injection, respectively. The preliminary tests enable to deduce on the influence of all factors, though they don't enable to assess the interaction between them. The results confirm literature information regarding the temperature and buffer concentration influence: the hydrolysis constant was higher at 34 ºC than at 17 ºC (pH 6.0, CT 500 mM) and at 500 mM than at 20 mM (17 ºC, pH 4.5). In addition to that, they point out to the unexpected possibility that the pressure increase is unfavorable to the hydrolysis constant. |
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| format | Online |
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| institution | Universidade Estadual de Campinas |
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| language | por |
| last_indexed | 2026-09-15T21:01:06Z |
| publishDate | 2016 |
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| spelling | ojs-article-89752026-09-15T14:04:57Z High pressure effect on the pharmaceutical substances efficiency Efeito de altas pressões na eficiência de substâncias farmacêuticas Carvalho, Priscila Hoffmann Ácido clavulânico. Estabilidade. Moléculas farmacêuticas. Some pathogenic bacteria manufacture -lactamases, enzymes that hydrolyze the antibiotic -lactam rings, such as penicillin and cephalosporin. Currently, the search for anti -lactam substances is still significantly important. Recent researches point out clavulanic acid (CA) as the main -lactam compound manufactured by Streptomyces clavuligerus. One of this biomolecule characteristics is the instability, due to the environment conditions where it is, affecting the purification yield. In this context, this work presents a CA stability study in different temperature (T), pH, buffer concentration (BC) and pressure (P) conditions. The CA solutions were submitted to different conditions for a defined time span and the concentrations were followed up, determining the answer, which is the hydrolysis constant (k). The experiments were performed in a cell with magnetic stirring and T and P controlled by thermostatically-controlled water bath and gaseous nitrogen injection, respectively. The preliminary tests enable to deduce on the influence of all factors, though they don't enable to assess the interaction between them. The results confirm literature information regarding the temperature and buffer concentration influence: the hydrolysis constant was higher at 34 ºC than at 17 ºC (pH 6.0, CT 500 mM) and at 500 mM than at 20 mM (17 ºC, pH 4.5). In addition to that, they point out to the unexpected possibility that the pressure increase is unfavorable to the hydrolysis constant. Algumas bactérias patogênicas, produzem -lactamases, enzimas que hidrolisam os anéis -lactâmicos dos antibióticos, como penicilina e cefalosporina. Atualmente, a busca por substâncias anti -lactamásicas ainda é significativamente expressiva. Pesquisas recentes apontam o ácido clavulânico (CA) como o principal composto -lactâmico produzido por Streptomyces clavuligerus. Uma característica dessa biomolécula é a sua instabilidade em função das condições do ambiente em que se encontra, afetando os rendimentos de purificação. Nesse contexto, o presente trabalho apresenta um estudo da estabilidade do CA em diferentes condições de temperatura (T), pH, concentração de tampão (CT) e pressão (P). As soluções de CA foram submetidas às diferentes condições por um intervalo de tempo definido e as concentrações foram acompanhadas, determinando-se a resposta, que é a constante de hidrólise (k). Os experimentos foram realizados em uma célula com agitação magnética e T e P controlados por banho termostatizado e injeção de nitrogênio gasoso, respectivamente. Os ensaios preliminares permitem inferir sobre a influência de cada um dos fatores, embora não permitam avaliar a interação entre eles. Os resultados confirmam as informações presentes na literatura a respeito da influência da temperatura e concentração de tampão: a constante de hidrólise foi maior a 34 ºC do que a 17 ºC (pH 6,0, CT 500 mM) e a 500 mM do que a 20 mM (17 ºC, pH 4,5). Além disso, apontam para a possibilidade inesperada de o aumento da pressão ser desfavorável ao aumento da constante de hidrólise. Universidade Estadual de Campinas (UNICAMP) 2016-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Análise lógica Analytical Research application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/8975 10.20396/simtec.v6.2016.8975 Resumo dos trabalhos do SIMTEC Simpósio dos Profissionais da UNICAMP; v. 6 (2016): Temática: UNICAMP 50 ANOS - Memórias, Experiências e Trajetórias profissionais; 144-144 2525-5398 por https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/8975/12559 Copyright (c) 2016 Priscila Hoffmann Carvalho https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Carvalho, Priscila Hoffmann Ácido clavulânico. Estabilidade. Moléculas farmacêuticas. High pressure effect on the pharmaceutical substances efficiency |
| title | High pressure effect on the pharmaceutical substances efficiency |
| title_full | High pressure effect on the pharmaceutical substances efficiency |
| title_fullStr | High pressure effect on the pharmaceutical substances efficiency |
| title_full_unstemmed | High pressure effect on the pharmaceutical substances efficiency |
| title_short | High pressure effect on the pharmaceutical substances efficiency |
| title_sort | high pressure effect on the pharmaceutical substances efficiency |
| topic | Ácido clavulânico. Estabilidade. Moléculas farmacêuticas. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/8975 |
| work_keys_str_mv | AT carvalhopriscilahoffmann highpressureeffectonthepharmaceuticalsubstancesefficiency AT carvalhopriscilahoffmann efeitodealtaspressoesnaeficienciadesubstanciasfarmaceuticas |
