Protocol establishment for micro propagation of b. portulacoides (amaranthaceae).
This work purpose is to establish protocol for vegetal tissue culture (important tool to preserve germoplasm and to maintain endangered species) with the species Blutaparon portulacoides (A. St.-Hil.) Mears, this species occurs between the upper high tide limit and the dunes and is endangered by the...
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| Language: | Portuguese |
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Universidade Estadual de Campinas (UNICAMP)
2016
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| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9778 |
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| author | Nunes, Viktor Krejci Salvador, Marcos José |
| author_facet | Nunes, Viktor Krejci Salvador, Marcos José |
| author_sort | Nunes, Viktor Krejci |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Resumo dos trabalhos do SIMTEC Simpósio dos Profissionais da UNICAMP; v. 6 (2016): Temática: UNICAMP 50 ANOS - Memórias, Experiências e Trajetórias profissionais; 196-196 |
| description | This work purpose is to establish protocol for vegetal tissue culture (important tool to preserve germoplasm and to maintain endangered species) with the species Blutaparon portulacoides (A. St.-Hil.) Mears, this species occurs between the upper high tide limit and the dunes and is endangered by the anthropic action. Therefore, the biolgical essay, biochemical procedures and vegetal biotechnology methodology adequacy will be proceeded. The vegetal material was collected in its natural habitat on the coastal area in two locations: Maricá (RJ) and Ubatuba (SP); after that, it was grown in a greenhouse, thus being a vegetal material source for the whole work. Explants (stem segments and most apical portions) were collected and submitted to in vitro experiments, they were distributed in 6 M&S culture means variations (MURASHIGE; SKOOG, 1962), for each variation had 30 repetitions; the piliferous zone development and the average length of main roots were assessed. With the treatment with 40g/L Sucrose and half of M&S standard means macronutrients was present among all groups that presented the best result within the experiment, being an alternative to this species micropropagation. |
| first_indexed | 2025-10-08T14:05:06Z |
| format | Online |
| id | ojs-article-9778 |
| institution | Universidade Estadual de Campinas |
| issn | 2525-5398 |
| language | por |
| last_indexed | 2026-09-21T21:01:53Z |
| publishDate | 2016 |
| publisher | Universidade Estadual de Campinas (UNICAMP) |
| record_format | ojs |
| spelling | ojs-article-97782026-09-21T13:22:48Z Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). Estabelecimento de protocolo para micropropagação de b. Portulacoides (amaranthaceae) Nunes, Viktor Krejci Salvador, Marcos José Germoplasma. Cultura de tecidos vegetais. Blutaparon portulacoides. This work purpose is to establish protocol for vegetal tissue culture (important tool to preserve germoplasm and to maintain endangered species) with the species Blutaparon portulacoides (A. St.-Hil.) Mears, this species occurs between the upper high tide limit and the dunes and is endangered by the anthropic action. Therefore, the biolgical essay, biochemical procedures and vegetal biotechnology methodology adequacy will be proceeded. The vegetal material was collected in its natural habitat on the coastal area in two locations: Maricá (RJ) and Ubatuba (SP); after that, it was grown in a greenhouse, thus being a vegetal material source for the whole work. Explants (stem segments and most apical portions) were collected and submitted to in vitro experiments, they were distributed in 6 M&S culture means variations (MURASHIGE; SKOOG, 1962), for each variation had 30 repetitions; the piliferous zone development and the average length of main roots were assessed. With the treatment with 40g/L Sucrose and half of M&S standard means macronutrients was present among all groups that presented the best result within the experiment, being an alternative to this species micropropagation. Objetiva-se neste trabalho o estabelecimento de protocolo para cultura de tecidos vegetais (importante ferramenta para conservação de germoplasma e para manutenção da espécie ameaçada) com a espécie Blutaparon portulacoides (A. St.-Hil.) Mears, esta espécie ocorre entre o limite superior da maré alta e as dunas e está ameaçada pela ação antrópica. Para tanto proceder-se-á adequação de metodologia dos ensaios biológicos e de procedimentos bioquímicos e da biotecnologia vegetal. O material vegetal foi coletado em seu habitat natural na faixa litorânea em duas localidades: Maricá (RJ) e Ubatuba (SP), em seguida foi cultivado em casa de vegetação, sendo assim uma fonte de material vegetal para todo o trabalho. Explantes (segmentos do caule e as porções mais apicais) foram coletados e submetidos a experimentos in vitro, eles foram distribuídos em 6 variações do meio de cultura M&S (MURASHIGE; SKOOG, 1962), para cada variação o experimento contou com 30 repetições, foram avaliados o desenvolvimento da zona pilífera e o comprimento médio das raízes principais. Sendo que o tratamento com 40g/L de Sacarose e metade dos macro-nutrientes do meio padrão M&S esteve presente entre todos os grupos que apresentaram melhor resultado dentro do experimento, sendo assim uma alternativa para a micropropagação desta espécie. Universidade Estadual de Campinas (UNICAMP) 2016-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Análise lógica Analytical Research application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9778 10.20396/simtec.v6.2016.9778 Resumo dos trabalhos do SIMTEC Simpósio dos Profissionais da UNICAMP; v. 6 (2016): Temática: UNICAMP 50 ANOS - Memórias, Experiências e Trajetórias profissionais; 196-196 2525-5398 por https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9778/12654 Copyright (c) 2016 Viktor Krejci Nunes, Marcos José Salvador https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Nunes, Viktor Krejci Salvador, Marcos José Germoplasma. Cultura de tecidos vegetais. Blutaparon portulacoides. Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| title | Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| title_full | Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| title_fullStr | Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| title_full_unstemmed | Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| title_short | Protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| title_sort | protocol establishment for micro propagation of b. portulacoides (amaranthaceae). |
| topic | Germoplasma. Cultura de tecidos vegetais. Blutaparon portulacoides. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9778 |
| work_keys_str_mv | AT nunesviktorkrejci protocolestablishmentformicropropagationofbportulacoidesamaranthaceae AT salvadormarcosjose protocolestablishmentformicropropagationofbportulacoidesamaranthaceae AT nunesviktorkrejci estabelecimentodeprotocoloparamicropropagacaodebportulacoidesamaranthaceae AT salvadormarcosjose estabelecimentodeprotocoloparamicropropagacaodebportulacoidesamaranthaceae |
