Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN)
This study’s goals were: (1) raise incidence and characterize the alloantibodies against erythrocytic antigens, in the serum from 2,126 pregnant women, served at CAISM, in the period from January 2014 to June 2015 and (2) investigate the maternal alloantibodies adherence in fetal red blood cells, in...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Online |
| Language: | Portuguese |
| Published: |
Universidade Estadual de Campinas (UNICAMP)
2016
|
| Subjects: | |
| Online Access: | https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9832 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1877067325214031872 |
|---|---|
| author | Pavani, Josymara Ming Locateli, Maria de Fátima Lieber, Sofia Rocha |
| author_facet | Pavani, Josymara Ming Locateli, Maria de Fátima Lieber, Sofia Rocha |
| author_sort | Pavani, Josymara Ming |
| collection | Portal de Eventos Científicos da UNICAMP |
| container_reference | Resumo dos trabalhos do SIMTEC Simpósio dos Profissionais da UNICAMP; v. 6 (2016): Temática: UNICAMP 50 ANOS - Memórias, Experiências e Trajetórias profissionais; 257-257 |
| description | This study’s goals were: (1) raise incidence and characterize the alloantibodies against erythrocytic antigens, in the serum from 2,126 pregnant women, served at CAISM, in the period from January 2014 to June 2015 and (2) investigate the maternal alloantibodies adherence in fetal red blood cells, in 76 samples from umbilical cord blood. Irregular antibodies in the maternal serum were investigated by the agglutination method in micro gel column, employing previously typed red blood cell panels (Diamed, USA). Maternal alloantibodies were checked in the fetal red blood cells, employing gel columns with polyclonal human antiglobulin and, after elution, by acid glycine or freezing and defrosting; then, their identification was undertaken. Erythrocytic sensitization was observed in 130 (6.1%) pregnant women, but only 85 (4.0%) carried clinically relevant antibodies for HDN. In 91.8% of the relevant cases, the incompatibility in the Rh system was the responsible for generating antibodies, highlighting the anti-D antibody (81.2%). Material antibodies joint to the fetal erythrocytes were observed in 18 (23.7%) cases. In 83.3%, they were directed to the Rh system antigens, exclusively, or associated to the A or B antigens. In 1 (5.6%) case, there was the identification of the anti-Fya antibody, related to the Duffy system. It was possible to conclude that, despite of the immunoprophylaxis, the allosensitization for D antigen is still relevant in our environment. In addition to that, it is necessary, along the pregnancy, to investigate the anti-A and anti-B antibodies, from the IgG class, as well as other systems, such as Duffy, with the purpose of minimize the HDN occurrence. |
| first_indexed | 2025-10-08T14:05:17Z |
| format | Online |
| id | ojs-article-9832 |
| institution | Universidade Estadual de Campinas |
| issn | 2525-5398 |
| language | por |
| last_indexed | 2026-09-21T21:02:07Z |
| publishDate | 2016 |
| publisher | Universidade Estadual de Campinas (UNICAMP) |
| record_format | ojs |
| spelling | ojs-article-98322026-09-21T14:57:14Z Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) Caracterização de anticorpos contra antígenos eritrocitários na doença hemolítica perinatal (DHPN) Pavani, Josymara Ming Locateli, Maria de Fátima Lieber, Sofia Rocha Doença hemolítica perinatal. Aloimunização materna. Incompatibilidade abo-rh. This study’s goals were: (1) raise incidence and characterize the alloantibodies against erythrocytic antigens, in the serum from 2,126 pregnant women, served at CAISM, in the period from January 2014 to June 2015 and (2) investigate the maternal alloantibodies adherence in fetal red blood cells, in 76 samples from umbilical cord blood. Irregular antibodies in the maternal serum were investigated by the agglutination method in micro gel column, employing previously typed red blood cell panels (Diamed, USA). Maternal alloantibodies were checked in the fetal red blood cells, employing gel columns with polyclonal human antiglobulin and, after elution, by acid glycine or freezing and defrosting; then, their identification was undertaken. Erythrocytic sensitization was observed in 130 (6.1%) pregnant women, but only 85 (4.0%) carried clinically relevant antibodies for HDN. In 91.8% of the relevant cases, the incompatibility in the Rh system was the responsible for generating antibodies, highlighting the anti-D antibody (81.2%). Material antibodies joint to the fetal erythrocytes were observed in 18 (23.7%) cases. In 83.3%, they were directed to the Rh system antigens, exclusively, or associated to the A or B antigens. In 1 (5.6%) case, there was the identification of the anti-Fya antibody, related to the Duffy system. It was possible to conclude that, despite of the immunoprophylaxis, the allosensitization for D antigen is still relevant in our environment. In addition to that, it is necessary, along the pregnancy, to investigate the anti-A and anti-B antibodies, from the IgG class, as well as other systems, such as Duffy, with the purpose of minimize the HDN occurrence. O presente estudo teve por objetivos: (1) levantar a incidência e caracterizar os aloanticorpos contra antígenos eritrocitários, no soro de 2126 gestantes, atendidas no CAISM, no período de janeiro de 2014 a junho de 2015 e (2) investigar a aderência dos alonticorpos maternos nas hemácias fetais, em 76 amostras de sangue de cordão umbilical. Anticorpos irregulares no soro materno foram investigados pelo método de aglutinação em microcoluna de gel, empregando-se painel de hemácias previamente tipadas (Diamed, USA). Aloanticorpos maternos nas hemácias fetais foram verificados empregando-se colunas de gel com antiglobulina humana policlonal e, após eluição, por glicina ácida ou congelamento e descongelamento, procedeu-se a identificação dos mesmos. Sensibilização eritrocitária foi observada em 130 (6,1%) gestantes, mas apenas 85 (4,0%) portavam anticorpos clinicamente relevantes para a DHPN. Em 91,8% dos casos relevantes, a incompatibilidade no sistema Rh foi a responsável na geração de anticorpos, com destaque para o anticorpo anti-D (81,2%). Anticorpos maternos aderidos aos eritrócitos fetais foram observados em 18 (23,7%) casos. Em 83,3%, eram dirigidos aos antígenos do sistema Rh, exclusivamente, ou associados aos antígenos A ou B. Em 1 (5,6%) caso, houve a identificação do anticorpo anti-Fya, relacionado ao sistema Duffy. Foi possível concluir que, apesar da imunoprofilaxia, a alosensibilização para o antígeno D ainda é relevante em nosso meio. Adicionalmente faz-se necessário, ao longo da gestação, a investigação de anticorpos anti-A e anti-B, da classe IgG, bem como, para outros sistemas como o Duffy, com o propósito de minimizar a ocorrência da DHPN. Universidade Estadual de Campinas (UNICAMP) 2016-10-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Análise lógica Analytical Research application/pdf https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9832 10.20396/simtec.v6.2016.9832 Resumo dos trabalhos do SIMTEC Simpósio dos Profissionais da UNICAMP; v. 6 (2016): Temática: UNICAMP 50 ANOS - Memórias, Experiências e Trajetórias profissionais; 257-257 2525-5398 por https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9832/12703 Copyright (c) 2016 Josymara Ming Pavani, Maria de Fátima Locateli, Sofia Rocha Lieber https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Pavani, Josymara Ming Locateli, Maria de Fátima Lieber, Sofia Rocha Doença hemolítica perinatal. Aloimunização materna. Incompatibilidade abo-rh. Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) |
| title | Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) |
| title_full | Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) |
| title_fullStr | Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) |
| title_full_unstemmed | Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) |
| title_short | Antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (HDN) |
| title_sort | antibody characterization against erythrocytic antigens on hemolytic disease of the newborn (hdn) |
| topic | Doença hemolítica perinatal. Aloimunização materna. Incompatibilidade abo-rh. |
| url | https://econtents.sbu.unicamp.br/eventos/index.php/simtec/article/view/9832 |
| work_keys_str_mv | AT pavanijosymaraming antibodycharacterizationagainsterythrocyticantigensonhemolyticdiseaseofthenewbornhdn AT locatelimariadefatima antibodycharacterizationagainsterythrocyticantigensonhemolyticdiseaseofthenewbornhdn AT liebersofiarocha antibodycharacterizationagainsterythrocyticantigensonhemolyticdiseaseofthenewbornhdn AT pavanijosymaraming caracterizacaodeanticorposcontraantigenoseritrocitariosnadoencahemoliticaperinataldhpn AT locatelimariadefatima caracterizacaodeanticorposcontraantigenoseritrocitariosnadoencahemoliticaperinataldhpn AT liebersofiarocha caracterizacaodeanticorposcontraantigenoseritrocitariosnadoencahemoliticaperinataldhpn |
